Water Chemistry Tests

Transparency

Background

Transparency is the measurement of water clarity. How clear the water is at your site will depend on the amount of soil particles suspended in the water and on the amount of algae or other growth at your site. Transparency may change seasonally with changes in growth rates, in response to precipitation runoff, or for other reasons. The clarity of your water determines how much light can penetrate. Since plants require light, transparency becomes an important measurement in determining productivity of your water site.

In the field you would measure transparency in one of two ways; with a Secchi disk in deep, still waters or with a turbidity tube if your site has shallow or running water. For the lab practice station, we will use the turbidity tube.

Temperature

Background

Water temperature is the temperature of a body of water such as a stream, river, pond, lake, well, or drainage ditch as it appears in nature. Water bodies can vary greatly in temperature, according to latitude, altitude, time of day, season, depth of water, and many other variables. Water temperature is important because it plays a key role in chemical, biological and physical interactions within a body of water. For example, high temperatures may be an indicator of increased plant production. The temperature of the water determines what aquatic plants and animals may be present since all species have their natural limits of tolerance to upper and lower temperatures. Water temperature can therefore help us to understand what may be happening within the water body without directly measuring hundreds of different things within the body of water.

 

Dissolved Oxygen

Background

All living things depend on oxygen to survive. In a water environment molecules of oxygen gas dissolve in the water. This is called dissolved oxygen (DO). In air, 20 out of every 100 molecules are oxygen. In water, only 1-5 molecules out of every million molecules are oxygen. This is why dissolved oxygen is measured in parts per million (ppm). Different species of aquatic organisms require different amounts of oxygen, but generally aquatic organisms require at least 6 ppm for normal growth and development.

Water temperature and altitude influence how much oxygen water can hold; i.e., the "equilibrium" value. In general, warmer water cannot hold as much oxygen as colder water. Similarly, at higher altitudes water cannot hold as much oxygen as waters at lower altitudes. Look for these patterns in the Temperature and Altitude Tables in the DO protocol. This is why we use a distilled water standard in the protocol and correct for temperature and altitude.

The actual amount of DO in a water may be higher or lower than the equilibrium value. Bacteria in the water consume oxygen as they digest decaying plant or animal materials. This can lower the DO levels of the water. In contrast, algae in lakes produce oxygen during photosynthesis which can sometimes result in higher DO levels in summer.

 

 


pH

Background

pH is an indicator of the acid content of water. The pH scale ranges from 1 (acid) to 14 (alkaline or basic) with 7 as neutral. The scale is logarithmic so a change of one pH unit means a tenfold change in acid or alkaline concentration. For instance, a change from 7 to 6 represents a solution 10 times more acidic; a change from 7 to 5 is 100 times more acidic, and so on. The lower the pH the more acidic the water. The pH of a water body has a strong influence on what can live in it. Immature forms of salamanders, frogs, and other aquatic life are particularly sensitive to low pH.

 

Alkalinity

Background

Alkalinity is a measure of the ability of a body of water to resist changes in pH when acids are added. Acid additions generally come from rain or snow, although soil sources may also be important in some areas. Alkalinity is generated when water dissolves rocks such as calcite and limestone. The alkalinity of natural waters protects fish and other aquatic organisms from sudden changes in pH.

 

Nitrate

Background

Nitrogen is one of the three major nutrients needed by plants. Most plants cannot use nitrogen in its molecular form (N2). In aquatic ecosystems blue-green algae are able to convert N2 into ammonia (NH3) and nitrate (NO3-) which can then be used by plants. Animals eat these plants to obtain nitrogen that they need to form proteins. When the plants and animals die, protein molecules are broken down by bacteria into ammonia. Other bacteria then oxidize the ammonia into nitrites (NO2-) and nitrates (NO3-). Under suboxic conditions nitrates can then be transformed by other bacteria into ammonia (NH3), beginning the nitrogen cycle again.

 

 

Typically nitrogen levels in natural waters are low (below 1 ppm nitrate nitrogen). Nitrogen released by decomposing animal excretions, dead plants, and animals is rapidly consumed by plants. In water bodies with high nitrogen levels eutrophication can occur. Nitrogen levels can become elevated from natural or human-related activities. Ducks and geese contribute heavily to nitrogen in the water where they are found. Man-made sources of nitrogen include sewage dumped into rivers, fertilizer washed into streams or leached into groundwater, and runoff from feedlots and barnyards.

Nitrate levels are measured in milligrams per liter nitrate nitrogen.

 

Phosphate

 

Background

 

Phosphates in some amount are necessary in all living things. Phosphates are found in various minerals. Calcium phosphate is found in shells, bones, and teeth. Plants must have phosphate to grow properly. This is why farmers and gardeners add fertilizers that contain phosphate to the soil. We use soaps and detergents for washing clothes, dishes, floors, and ourselves. Soaps, detergents, and other cleaners often contain  phosphates. Phosphates are powerful cleaners. Too much phosphate in water is usually the result of human activity. Plants absorb some of the phosphate in fertilizer put on farms and lawns. Any unused phosphates may be carried to a stream, creek, pond, or river by runoff.

 

 

 

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